                         SEQUENCE LISTING

<110>  Ben-Gurion University of the Negev Research and Development
        Authority
 
<120>  CHEMICAL PREPARATION OF POLYUBIQUITIN CHAINS

<130>  11-069PCT

<140>  PCT/IL2011/000137
<141>  2011-02-09

<150>  US 61/302,559
<151>  2010-02-09

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  2
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized ubiquitin with the N-terrminus methionine replaced by
       leucine.

<400>  2

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  3
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The C-terminus residue of this artificial sequence is linked to a
       thioester group, -SR, whereiin R is an alkyl or aryl group.

<400>  3

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45  


<210>  4
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The C-terminus residue of this artificial sequence is linked to a
       thioester group, -SR, wherein R is an alkyl or aryl group.


<220>
<221>  X
<222>  (33)..(33)
<223>  X is thiazolidine-protected mercaptolysine

<400>  4

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Xaa Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45  


<210>  5
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The C-terminus residue of this artificial sequence is linked to a
       thioester group, -SR, whereiin R is an alkyl or aryl group.


<220>
<221>  X
<222>  (29)..(29)
<223>  X is thiazolidine-protected mercaptolysine

<400>  5

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Xaa Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45  


<210>  6
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The C-terminus residue of this artificial sequence is linked to a
       thioester group, -SR, whereiin R is an alkyl or aryl group.


<220>
<221>  X
<222>  (27)..(27)
<223>  X is thiazolidine-protected mercaptolysine

<400>  6

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Xaa Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45  


<210>  7
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The C-terminus residue of this artificial sequence is linked to a
       thioester group, -SR, wherein R is an alkyl or aryl group.


<220>
<221>  X
<222>  (11)..(11)
<223>  X is thiazolidine-protected mercaptolysine

<400>  7

Leu Gln Ile Phe Val Lys Thr Leu Thr Gly Xaa Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45  


<210>  8
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The C-terminus residue of this artificial sequence is linked to a
       thioester group, -SR, wherein R is an alkyl or aryl group.


<220>
<221>  X
<222>  (6)..(6)
<223>  X is thiazolidine-protected mercaptolysine

<400>  8

Leu Gln Ile Phe Val Xaa Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe 
        35                  40                  45  


<210>  9
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminus fragment of ubiquitin

<400>  9

Cys Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
1               5                   10                  15      


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
            20                  25                  30      


<210>  10
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminus fragment of modified ubiquitin


<220>
<221>  X
<222>  (18)..(18)
<223>  X is thiazolidine-protected mercaptolysine

<400>  10

Cys Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
1               5                   10                  15      


Gln Xaa Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
            20                  25                  30      


<210>  11
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminus fragment of modified ubiquitin


<220>
<221>  X
<222>  (3)..(3)
<223>  X is thiazolidine-protected mercaptolysine

<400>  11

Cys Gly Xaa Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
1               5                   10                  15      


Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
            20                  25                  30      


